Introduction to r-DNA Technology, Fundamental methods: precipitation of nucleic acids, determination of concentration of nucleic acids, purification of nucleic acids, Restriction enzymes, electrophoresis gels, blotting techniques, Polymerase chain reaction and its applications, Basics of cloning, introduction to cloning process, Vectors for cloning: plasmids, bacteriphages, cosmids, P1 vectors, PAC, BAC, YAC, MAC, DNA sequencing techniques, Methods for analyzing DNA for mutations, Expression systems: bacterial, yeast, baculoviral, mammalian, Advances in r-DNA technology