Genetic Engineering (CH862)

Programme: 

M.Tech (IBT)

Category: 

Elective Courses (Ele)

Credits (L-T-P): 

03 (3-0-0)

Content: 

Introduction to r-DNA Technology, Fundamental methods: precipitation of nucleic acids, determination of concentration of nucleic acids, purification of nucleic acids, Restriction enzymes, electrophoresis gels, blotting techniques, Polymerase chain reaction and its applications, Basics of cloning, introduction to cloning process, Vectors for cloning: plasmids, bacteriphages, cosmids, P1 vectors, PAC, BAC, YAC, MAC, DNA sequencing techniques, Methods for analyzing DNA for mutations, Expression systems: bacterial, yeast, baculoviral, mammalian, Advances in r-DNA technology

References: 

Molecular Biology and Genomics, CORNEL M√úLHARDT, The Experimenter series, Academic press,
Gene cloning and DNA analysis, T.A. Brown, sixth edition ,Wiley- Blackwell
Principles of Gene manipulation and genomics, S. Primrose, Wiley, second edition

Department: 

Chemical Engineering
 

Contact us

Prasanna B D, Associate Professor and Head
Department of Chemical Engineering, NITK, Surathkal
P. O. Srinivasnagar, Mangalore - 575 025
Karnataka, India.

  • Hot line: +91-0824-2474057

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