Introduction: Design and construction of novel proteins and enzymes, Conformation of proteins in general and enzymes in particular, Effect of amino acids on structure of proteins, Energy status of a protein molecule, Structure function relations of enzymes, Physical methods such as x-ray crystallography for determination of protein structure, Site directed mutagenesis for specific protein function, Basic concepts for design of a new protein/enzyme molecule, Specific examples of enzyme engineering, -Tryesyl t RNAsynthetase, Dihydrofolate reductase, Subtilisin.